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| Title:
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Application of bipolar electrodialysis to E.coli fermentation for simultaneous acetate removal and pH control |
| Type:
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Journal articleJournal article |
| Participant(s):
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Forfatter:
Wong, M.
Technical University of Denmark
Technical University of Denmark
Email:
Forfatter:
Lye, G.J.
Technical University of Denmark
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| Abstract:
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The application of bipolar electrodialysis (BPED) for the simultaneous removal of inhibitory acetate and pH control during E. coli fermentation was investigated. A two cell pair electrodialysis module, consisting of cation exchange, anion exchange and bipolar membranes with working area of 100 cm2 each, was integrated with a standard 7 l stirred tank bioreactor. Results showed that BPED was beneficial in terms of in situ removal of inhibitory acetate and a reduction in the amount NH4OH used for pH control. In batch and fed-batch BPED fermentations, base additions were decreased by up to 50% in both cases compared to electrodialysis (ED) fermentations with pH controlled at 6.7 ± 0.1. Consequently, the final biomass (34.2 g DCW l−1) and recombinant protein (5.5 g l−1) concentrations obtained were increased by up to 37 and 20%, respectively. |
| Published:
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in journal: Biotechnology Letters (ISSN: 0141-5492) (DOI: http://dx.doi.org/10.1007/s10529-010-0266-0), vol: 32, issue: 8, pages: 1053-1057, 2010 |
| DOI:
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